Nutritional requirements for synthesis of heat-stable enterotoxin by Yersinia enterocolitica
نویسندگان
چکیده
منابع مشابه
Detection of Yersinia enterocolitica heat-stable enterotoxin by suckling mouse bioassay.
A comparison of heat-stable enterotoxins from Escherichia coli and Yersinia enterocolitica by the suckling mouse bioassay showed that whereas E. coli heat-stable enterotoxin evoked a maximum ratio of gut weight to remaining body weight after a 4-h incubation period, the maximum ratio for Y. enterocolitica was achieved at 2 h, decreasing thereafter to values regarded as negative (less than 0.083...
متن کاملPartial purification and characterization of heat-stable enterotoxin produced by Yersinia enterocolitica.
By using a suckling mouse assay, heat-stable enterotoxin (ST) was purified from the culture filtrate of Yersinia enterocolitica isolated from a diarrheal patient. The purification procedures involve ultrafiltration with an Amicon HIP-10 hollow fiber, ethanol fractionation, protamine sulfate treatment, diethylaminoethyl-Sephacel and hydroxylapatite column chromatographies, and Sephacryl S-200 su...
متن کاملFactors affecting detection of Yersinia enterocolitica heat-stable enterotoxin by the infant mouse test.
With regard to the assay of heat-stable enterotoxin (ST) from Yersinia enterocolitica, we made a comparative study of the conventional infant mouse test read at 4 h and a modified infant mouse test read at 2 h. The influence of several factors, such as the medium used to prepare ST, lysing of bacterial cells from the broth cultures used to prepare ST, and the temperature at which the inoculated...
متن کاملProduction of enterotoxin by Yersinia enterocolitica.
Forty-three strains of Yersinia enterocolitica isolated from children with gastroenteritis and 18 laboratory strains were examined for enterotoxin production by using the infant mouse, Y1 adrenal cell, and rabbit ileal loop assay systems. All clinical isolates and seven laboratory strains were found enterotoxigenic in the infant mouse model, but none of the strains produced toxin in the Y1 adre...
متن کاملproduction and purfication of heat-stable (st) enterotoxin of yersinia enterocolitica
since the heat-stable enterotoxin (st) produced by yersinia enterocolitica is similar to enterotoxin production by e. coli, it is probably classified among toxinogenic bacteria. the culture filtrate of st-producing strains of y. enterocolitica is able to activate guanylate cyclase and to increase fluid accumulation action of cylic guanosin 3', 5' - monophosphate (cgmp) in sucking mous...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1993
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.59.10.3314-3320.1993